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Calibration Curve Preparation

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Preparation of Calibration Curve Aim: To prepare calibration curve for given sample of drug. Reference: https://papcsg.blogspot.com/2018/12/calibration-curve-preparation.html Principle:  Every molecule have a transition state either n, 𝛑, 𝛔 ..etc. Molecules have a constant point to absorb energy and get energized, as a result they change their transition state.  On increasing the concentration the absorption is also enhanced. These result help to create a standard linear equation.  Solution are prepared of different concentration and analysed, the absorption peak help to create a graph. After preparation of graph a straight linear equation determined as:    y = mx + c  Requirements: Chemical : Drug, Solvent (as standard) Apparatus : U V spectrometer, weighing  machine Beaker, Volumetric Flask  100 and 10 ml, Pipette. Procedure: * Preparation of stock solution (100 ppm): Weigh out 10 mg of drug and dissolve in...
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Hydrolysis of Starch by Salivary Amylase - PAPC

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   Biochemistry and Clinical Pathology -Practical PAPC Aim: To study hydrolysis of Starch from salivary amylase. Reference: 1. R ao. G. Devela. ‘A Manual of Practical Biochemistry’. Birla Publication. PVT LTD. Delhi. Second Edition. 2008. 04-10. 2. Chaudhari M.R. et.al. ‘Biochemistry and Clinical Pathology’. Nirali Prakashan. Pune. Thirty Fourth Edition. 2018. 3.1 – 3.14. 3. Indian Pharmacopeia. ' Ministry of Health and Family Welfare'. Indian Pharmacopeia Commission. Gaziabad. Vol I. 888. Requirement: Chemical: Strach, Salivary amylase diluted, 0.1N Iodine Solution, Acetic Acid, Hydrochloric Acid, Sodium Hydroxide, Phosphate buffer 6.8 pH, Distill Water etc. Apparatus: Test Tube, Beaker, Water Bath, Watch Glass, Test Tube Holder, Glass Rod. Principle: Enzyme are responsible to regulate rate of reaction and Amylase break down Starch to simple sugar. On digestion of starch chracterstic inference will not be observed. Procedure: > Take 4 test tube with 1 ml of 1 % Starch so...
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Percentage Drug Content

Determination of Percentage Drug Content Aim: To calculate percentage drug content for given sample of drug (marketed preparation). Reference: https://papcsg.blogspot.com/2018/12/determination-of-percentage-drug.html Theory: Different concentration of a standard Drug solution, when analysed in U V spectrometer at a wave length as given in reference book, drug solution provide a Absorption peak.   Different peaks help to create a Standard Calibration Curve, this is helpful in determining Percentage Drug Content . Calibration Curve find as: y = mx + c. Where,  y =  absorption peak, x = concentration, m = slope and c = intercept point.   on putting of value of y, x can easily be determined. Requirement: Chemical: Marketed Formulation and Solvent Equipment: U V Spectrometer and Weighing Machine Apparatus: Beaker, Volumetric flask and Pipette. Procedure: * Preparation of Test Solution: 1. Weigh out 100 mg of powd...
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Benzanilide from Aniline

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Synthesis of Benzanilide from Aniline Aim:  To synthesize Benzanilide from Aniline, calculate its melting point and percentage yield. Reference:   https://papcsg.blogspot.com/2018/12/benzanilide-from-aniline.html Principle:  Insertion of benzoyl to aniline moiety termed as benzoylation reaction. 10 % NaOH added to neutralize HCl and it also increase the rate of reaction. Requirements: Chemical: Aniline 3 ml, Benzoyl Chloride 6 ml, 10% NaOH 45 ml, Ethanol 6 ml, Cold Water 10 ml  Apparatus: Weighing Machine, M. P. apparatus, Conical Flask - 250 ml, Measuring Cylinder, Funnel, Filter Paper, Poly Bag Procedure: 1. Add Aniline and NaOH in to a conical flask. Dissolve it completely. 2. Add Benzoyl Chloride in to flask. 3. Heat it on boiling water bath for 15 minutes. 4. Add cold water in to the preparation, and a solid was formed. 5. Filter it and recrystallized it using ethanol.  6. Weight and Melting Point w...
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