Calibration Curve Preparation

Preparation of Calibration Curve

Aim: To prepare calibration curve for given sample of drug.

Reference: https://papcsg.blogspot.com/2018/12/calibration-curve-preparation.html

Principle: 
Every molecule have a transition state either n, 𝛑, 𝛔 ..etc. Molecules have a constant point to absorb energy and get energized, as a result they change their transition state. 
On increasing the concentration the absorption is also enhanced. These result help to create a standard linear equation. 
Solution are prepared of different concentration and analysed, the absorption peak help to create a graph.
After preparation of graph a straight linear equation determined as:
   y = mx + c 

Requirements:
Chemical : Drug, Solvent (as standard)
Apparatus : U V spectrometer, weighing  machine
Beaker, Volumetric Flask  100 and 10 ml, Pipette.

Procedure:
* Preparation of stock solution (100 ppm):
Weigh out 10 mg of drug and dissolve in 100 ml of solvent in a 100 mlml of volumetric flask.

* Preparation of  test solution:
1. Take 0.1 ml from stock solution and make up to 10 ml using solvent in 10 ml of volumetric flask.
Solution was 1 ppm.
2. Take 0.2 ml from stock solution and make up to 10 ml using solvent in 10 ml of volumetric flask.
Solution was 2 ppm.
3. Take 0.3 ml from stock solution and make up to 10 ml using solvent in 10 ml of volumetric flask.
Solution was 3 ppm.
4. Take 0.4 ml from stock solution and make up to 10 ml using solvent in 10 ml of volumetric flask.
Solution was 4 ppm.
5. Take 0.5 ml from stock solution and make up to 10 ml using solvent in 10 ml of volumetric flask.
Solution was 5 ppm.
Fill out the cubate with solvent and correct base line and start analysis.

* Preparation of Calibration Curve:
Using observation table a curve was prepared, and straight line equation calculated by using Microsoft Word.

Observation table:

Concentration
Absorbance
1 ppm
A1
2 ppm
A2
3 ppm
A3
4 ppm
A4
5 ppm
A5

Calibration Curve:
A Curve shows as:
Result:
Calibration curve was prepared for given sample of drug.
Straight line equation was : y = mx + c and value of R2 was: x.


 Source:
→https://en.wikipedia.org/wiki/Calibration_curve 26/12/2018
→Jain A. 'Pharmaceutical Instrumental Analysis'. Nirali Prakashan. Pune. Third Edition. 2017.
→Chatwal G. R. Ahmed S. K. 'Instrumental Chemical Analysis'. Himalaya Publication PVT LTD. Mumbai. Edition 2008.

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