Experiments List

Comments

Post a Comment

Popular posts from this blog

Sennoside Isolation

Image
Isolation of Sennosides from senna leaves Aim: To isolate sennoside from senna leaves. Reference: https://papcsg.blogspot.com/2018/12/sennoside-isolation.html Theory:  Biological Source: Senna obtain from leaves of plant Cassia angustifolia and C. accutifolia belongs to family: Leguminoceae. Senna contain mainly chemicals such as anthraquinones, anthrone and dianthrone. Other chemicals such as sennosides A and B, present in at least 2.5% concentration. It mainly used for purgative action in condition of constipation. Soxhlet apparatus used for continuous extraction process and may used for direct heat sensitive drug. The process of extraction is continuous so, it is also known as Continuous Hot Percolation method.  Requirements: > Senna Powder Leaves (50 gm) > Solvent (Distill water 250 ml) > Soxhlet Assembly > Weighing machine > Heating Metal > Evaporator > Beaker, Piece of Cotton, Filter Paper Procedure: ...
Read more

Percentage Drug Content

Determination of Percentage Drug Content Aim: To calculate percentage drug content for given sample of drug (marketed preparation). Reference: https://papcsg.blogspot.com/2018/12/determination-of-percentage-drug.html Theory: Different concentration of a standard Drug solution, when analysed in U V spectrometer at a wave length as given in reference book, drug solution provide a Absorption peak.   Different peaks help to create a Standard Calibration Curve, this is helpful in determining Percentage Drug Content . Calibration Curve find as: y = mx + c. Where,  y =  absorption peak, x = concentration, m = slope and c = intercept point.   on putting of value of y, x can easily be determined. Requirement: Chemical: Marketed Formulation and Solvent Equipment: U V Spectrometer and Weighing Machine Apparatus: Beaker, Volumetric flask and Pipette. Procedure: * Preparation of Test Solution: 1. Weigh out 100 mg of powd...
Read more

Hydrolysis of Starch by Salivary Amylase - PAPC

Image
   Biochemistry and Clinical Pathology -Practical PAPC Aim: To study hydrolysis of Starch from salivary amylase. Reference: 1. R ao. G. Devela. ‘A Manual of Practical Biochemistry’. Birla Publication. PVT LTD. Delhi. Second Edition. 2008. 04-10. 2. Chaudhari M.R. et.al. ‘Biochemistry and Clinical Pathology’. Nirali Prakashan. Pune. Thirty Fourth Edition. 2018. 3.1 – 3.14. 3. Indian Pharmacopeia. ' Ministry of Health and Family Welfare'. Indian Pharmacopeia Commission. Gaziabad. Vol I. 888. Requirement: Chemical: Strach, Salivary amylase diluted, 0.1N Iodine Solution, Acetic Acid, Hydrochloric Acid, Sodium Hydroxide, Phosphate buffer 6.8 pH, Distill Water etc. Apparatus: Test Tube, Beaker, Water Bath, Watch Glass, Test Tube Holder, Glass Rod. Principle: Enzyme are responsible to regulate rate of reaction and Amylase break down Starch to simple sugar. On digestion of starch chracterstic inference will not be observed. Procedure: > Take 4 test tube with 1 ml of 1 % Starch so...
Read more